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| Registros recuperados: 181 | |
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| Soares,Taís Cristina Bastos; Good-God,Pedro Ivo Vieira; Miranda,Fábio Demolinari de; Soares,Janaína Bastos; Schuster,Ivan; Piovesan,Newton Deniz; Barros,Everaldo Gonçalves de; Moreira,Maurilio Alves. |
| The objectives of this study were to detect quantitative trait loci (QTL) for protein content in soybean grown in two distinct tropical environments and to build a genetic map for protein content. One hundred eighteen soybean recombinant inbred lines (RIL), obtained from a cross between cultivars BARC 8 and Garimpo, were used. The RIL were cultivated in two distinct Brazilian tropical environments: Cascavel county, in Paraná, and Viçosa county, in Minas Gerais (24º57'S, 53º27'W and 20º45'S, 42º52'W, respectively). Sixty-six SSR primer pairs and 65 RAPD primers were polymorphic and segregated at a 1:1 proportion. Thirty poorly saturated linkage groups were obtained, with 90 markers and 41 nonlinked markers. For the lines cultivated in Cascavel, three QTL... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Glycine max; Linkage map; Molecular markers; Recombinant inbred lines; Quantitative trait loci. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2008001100012 |
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| Ferreira,Marcia Flores da Silva; Cervigni,Gerardo Domingo Lucio; Ferreira,Adésio; Schuster,Ivan; Santana,Fernanda Abreu; Pereira,Waldir Dias; Barros,Everaldo Gonçalves de; Moreira,Maurilio Alves. |
| The objective of this work was to identify major and minor-effect quantitative trait loci (QTL) for resistance to races 3, 9, and 14 of soybean cyst nematode (SCN) in Hartwig cultivar; to map new resistance QTLs for these races; and to check for the existence of epistatic interactions between QTLs. Cultivar Hartwig is an important resistance source to SCN. Recombinant inbred lines (RIL) obtained from a cross between 'Hartwig' (resistant) and Y23 (susceptible) were evaluated regarding resistance to the three races. New genomic regions for resistance to SCN were identified by microsatellites. Four QTLs, which explained between 12 and 34% of phenotypic variance, were detected for resistance to race 3 in linkage groups (LG) A2, G, J, and M. The QTL in LG G is... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Glycine max; Breeding; Marker-assisted selection; Molecular markers. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2011000400012 |
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| Cabral,Pablo Diego Silva; Soares,Taís Cristina Bastos; Gonçalves,Leandro Simões Azeredo; Amaral Júnior,Antonio Teixeira do; Lima,Andreia Barcelos Passos; Rodrigues,Rosana; Matta,Frederico de Pina. |
| The present work aimed at evaluating the divergence among common bean accessions by their agronomic, morphological and molecular traits, based on the Ward-MLM procedure. A collection of 57 accessions from the gene bank of Universidade Federal do Espírito Santo was used in this study, from which: 31 were landraces belonging to the community Fortaleza, in the municipality of Muqui, ES, Brazil; 20 accessions were provided by Embrapa Trigo; and 6 were commercial cultivars. Five agronomic traits (plant cycle, number of seeds per pod, number of pods per plant, weight of 100 seeds, and grain yield), five morphological traits (growth habit, plant size, seed shape, seed color, and commercial group) and 16 microsatellite primers were evaluated. High genetic... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Phaseolus vulgaris; Genetic diversity; Germplasm characterization; Joint analysis; Molecular markers; Morphological markers. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2010001000011 |
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| Miyata,Marcelo; Gasparin,Gustavo; Coutinho,Luiz Lehmann; Martinez,Mario Luiz; Machado,Marco Antonio; Silva,Marcos Vinicius G. Barbosa da; Campos,Ana Lucia; Sonstegard,Tad S.; Rosário,Millor Fernandes do; Regitano,Luciana Correia de Almeida. |
| Quantitative trait loci (QTL) mapping in livestock allows the identification of genes that determine the genetic variation affecting traits of economic interest. We analyzed the birth weight and weight at 60 days QTL segregating on bovine chromosome BTA14 in a F2 resource population using genotypes produced from seven microsatellite markers. Phenotypes were derived from 346 F2 progeny produced from crossing Bos indicus Gyr x Holstein Bos taurus F1 parents. Interval analysis to detect QTL for birth weight revealed the presence of a QTL (p < 0.05) at 1 centimorgan (cM) from the centromere with an additive effect of 1.210 ± 0.438 kg. Interval analysis for weight at 60 days revealed the presence of a QTL (p < 0.05) at 0 cM from the centromere with an... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: TA14; Cattle; F2; Growth traits; Molecular markers; QTL. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000300011 |
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| Fernández R.,Marta; Valenzuela A.,Sofía; Balocchi L.,Claudio. |
| Eucalyptus globulus is the second most important forest species in Chile, after Pinus radiata. The main advantages of E. globulus are its fast growth (25 m³/ha/year) and its excellent wood quality for kraft pulp production. On the negative side, its low freezing tolerance has been an obstacle for the expansion of plantations, specifically on the foothills of the Andes. The difference in the freezing resistance between clones of E. globulus has a genetic base and, therefore, it could be detected through DNA molecular markers. Fifteen clones of E. globulus, eight classified as freezing resistant and seven as freezing sensitive were analyzed using the technique of RAPD, in order to obtain molecular markers that could differentiate between freezing sensitive... |
| Tipo: Journal article |
Palavras-chave: Eucalyptus globulus; Freezing resistance; Molecular markers; RAPD. |
| Ano: 2006 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000300025 |
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| Martins Filho,Sebastião; Sediyama,Carlos Sigueyuki; Moreira,Maurilio Alves; Barros,Everaldo Gonçalves de. |
| The soybean (Glycine max (L.) Merrill) frogeye leaf spot is caused by the fungus Cercospora sojina Hara and is a widespread disease in Brazil and other countries, causing severe losses in grain yield and also affecting seed quality. The availability of DNA markers linked to genes for resistance to this disease would accelerate breeding programs, particularly when other traits are also being evaluated. Bulked segregant analysis was applied to 3 F2 populations derived from crosses between the resistant cultivars Parana, Cristalina and Uberaba, and the susceptible cultivar Bossier. In the cross 'Parana' x 'Bossier', 2 RAPD markers were identified, CSOPA1(800C) and CSOPA2(1,250C), located at 4.4 ± 1.8 centiMorgans (cM) and 3.4 ± 1.7 cM respectively from the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Molecular markers; RAPD; SCAR; Soybean; Cercospora sojina; Frogeye leaf spot. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000300012 |
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| Garcia Meunier, Pascale; Martel, Corine; Pigeot, Jacques; Chevalier, Guillemette; Blanchard, Gérard; Goulletquer, Philippe; Robert, Stephane; Sauriau, Pierre-guy. |
| The direct amplification of length polymorphism technique (DALP) has been used to distinguish species-specific banding patterns in two marine gastropod oyster drills Ocenebra erinacea (Linnaeus, 1758) and Ocinebrellus inornatus (Recluz, 1851). Ocenebra erinacea is the European oyster drill, common along all European coasts. Ocinebrellus inornatus, the Japanese oyster drill, was recorded in oyster growing areas of the Marennes-Oleron Bay (SW France) for the first time in 1995. This new biological invasion could lead to an increase, which must be evaluated, in the predation risk for cultivated species i.e. oysters and blue mussels, and for littoral fishing resources along the French Atlantic coasts. As a result, since specific identification of early life... |
| Tipo: Text |
Palavras-chave: French Atlantic coast; Ocenebra erinacea; Ocinebrellus inornatus; Marine bioinvasions; Molecular markers. |
| Ano: 2002 |
URL: http://archimer.ifremer.fr/doc/2002/publication-569.pdf |
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| Almeida,Dênia Pires de; Castro,Isabel Samila Lima; Mendes,Tiago Antônio de Oliveira; Alves,Danúbia Rodrigues; Barka,Geleta Dugassa; Barreiros,Pedro Ricardo Rossi Marques; Zambolim,Laércio; Sakiyama,Ney Sussumu; Caixeta,Eveline Teixeira. |
| ABSTRACT: The biotrophic fungus Hemileia vastatrix causes coffee leaf rust (CLR), one of the most devastating diseases in Coffea arabica . Coffee, like other plants, has developed effective mechanisms to recognize and respond to infections caused by pathogens. Plant resistance gene analogs (RGAs) have been identified in certain plants as candidates for resistance ( R ) genes or membrane receptors that activate the R genes. The RGAs identified in different plants possess conserved domains that play specific roles in the fight against pathogens. Despite the importance of RGAs, in coffee plants these genes and other molecular mechanisms of disease resistance are still unknown. This study aimed to sequence and characterize candidate genes from coffee plants... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Coffea arabica; Coffee leaf rust; Resistance gene analogs; Molecular markers; Plant breeding. |
| Ano: 2021 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162021000601101 |
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| Pinto,Luciana Rossini; Vieira,Maria Lucia Carneiro; Souza Jr.,Claudio Lopes de; Souza,Anete Pereira de. |
| A modified reciprocal recurrent selection (RRS) method, which employed one cycle of high-intensity selection, was applied to two tropical maize (Zea mays L.) populations, BR-105 and BR-106, originating the improved synthetics IG-3 and IG-4, respectively. In the present study the effects of this kind of selection on the genetic structure of these populations and their synthetics were investigated at 30 microsatellite (SSR) loci. A total of 125 alleles were revealed. A reduction in the number of alleles was observed after selection, as well as changes in allele frequencies. In nearly 13% (BR-105) and 7% (BR-106) of the loci evaluated, the changes in allele frequencies were not explained, exclusively due to the effects of genetic drift. The effective... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Population genetics; Plant breeding; Molecular markers. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000300022 |
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| Caixeta,Débora Santos; Fritsche-Neto,Roberto; Batista,Lorena Guimarães; Carvalho,Humberto Fanelli; DoVale,Júlio César; Lanes,Éder Cristian Malta de; Miranda,Glauco Vieira. |
| The objective of this study was to determine the relationship between heterosis and genetic divergence for phosphorus use efficiency (PUE) in tropical maize. It was used two groups of genitors, each consisting of seven lines, contrasting with each other in the nitrogen and phosphorus use efficiency. It was obtained 41 hybrid combinations between these groups, which were evaluated in low phosphorus. Randomized complete block design with two replications was used. For obtaining the components of variance and the breeding values were used REML/BLUP method. In the genotyping of the parental lines were used 80 microsatellite markers. Through the correlation between genetic distance obtained by the markers and specific combining ability it was not possible to... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Dissimilarity; Abiotic stress; Molecular markers; Combining ability. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782013000100011 |
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| Podio,Maricel; Rodríguez,María P.; Felitti,Silvina; Stein,Juliana; Martínez,Eric J.; Siena,Lorena A.; Quarin,Camilo L.; Pessino,Silvina C.; Ortiz,Juan Pablo A.. |
| In previous studies we reported the identification of several AFLP, RAPD and RFLP molecular markers linked to apospory in Paspalum notatum. The objective of this work was to sequence these markers, obtain their flanking regions by chromosome walking and perform an in silico mapping analysis in rice and maize. The methylation status of two apospory-related sequences was also assessed using methylation-sensitive RFLP experiments. Fourteen molecular markers were analyzed and several protein-coding sequences were identified. Copy number estimates and RFLP linkage analysis showed that the sequence PnMAI3 displayed 2-4 copies per genome and linkage to apospory. Extension of this marker by chromosome walking revealed an additional protein-coding sequence mapping... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Apomixis; Chromosome walking; Gene mapping; Molecular markers. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000500018 |
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| Menezes,Carlos C.E; Vantoai,Tara; McDonald,Miller B; Sediyama,Tocio. |
| Sequence-Characterized Amplified Region (SCAR) appears as a useful technique for genetic purity testing and variety discrimination, applicable to species in which some other techniques have failed. In particular, this technique is very attractive with species in which RAPD results were not consistent. The RAPD polymorphic bands were cloned, sequenced and from the sequence information, primers pairs for normal PCR were developed. Since the probability of obtaining successful SCAR primers from RAPD polymorphic bands was about 50%, a larger number of RAPD polymorphic bands are needed to develop sufficient SCAR primers for varietal discrimination in vinca. In addition, the efficiency of the SCAR technique is strongly affected by the quality of DNA extracted... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Vinca; Seed; Variety discrimination; Molecular markers; SCAR. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-31222002000100041 |
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| Silva,Luciano da Costa e; Cruz,Cosme Damião; Moreira,Maurilio Alves; Barros,Everaldo Gonçalves de. |
| Various population sizes and number of markers have been used to obtain genetic maps. However, the precise number of individuals and markers needed for obtaining reliable maps is not known. We used data simulation to determine the influence of population size, the effect of the degree of marker saturation of the genome, and the number of individuals required for mapping of recombinant inbred lines (RILs). Three genomes with 11 linkage groups were generated with saturation levels of 5, 10 and 20 cM. For each saturation level populations were generated with 50, 100, 154, 200, 300, 500 and 800 individuals with 100 replications for each population size. A total of 2100 populations was generated and mapped. Small marker numbers and small population sizes... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Recombinant inbred lines; Molecular markers; Number of individuals; Computer simulation. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000600013 |
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| González,Germán Andrés; Pacheco,María Gabriela; Oneto,Cecilia Décima; Etchart,Valeria J; Kandus,Mariana V; Salerno,Juan Carlos; Eyherabide,Guillermo; Presello,Daniel; Lewi,Dalia Marcela. |
| Somatic embryogenesis, which is still the method of choice for tissue culture, regeneration and transformation of maize, is largely considered highly genotype-dependent. The Hi II, a highly embryogenic genotype, has been extensively used in transformation protocols. However, this is not an inbred line; instead, it has a proportion of the undesirable A-188 background, and the progeny segregates for phenotypic characteristics and shows poor agronomic performance. In an effort to identify genotypes that combine a high somatic embryogenic response and good agronomic performance, we evaluated 48 advanced inbred lines developed at INTA. Callus development and somatic embryogenesis capacity were measured in 200 immature embryos per line. Embryogenic capacity [EC... |
| Tipo: Journal article |
Palavras-chave: Callus; Inbred lines; Maize; Molecular markers; Regeneration; Somatic embryogenesis. |
| Ano: 2012 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000100009 |
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| Santos,Carlos A.F.; Simon,Philipp W.. |
| Amplified fragment length polymorphism (AFLP) is a fast and reliable tool to generate a large number of DNA markers. In two unrelated F2 populations of carrot (Daucus carota L.), Brasilia x HCM and B493 x QAL (wild carrot), it was hypothesized that DNA 1) digested with the same restriction endonuclease enzymes and amplified with the same primer combination and 2) sharing the same position in polyacrylamide gels should be conserved sequences. To test this hypothesis AFLP fragments from polyacrylamide gels were eluted, reamplified, separated in agarose gels, purified, cloned and sequenced. Among thirty-one paired fragments from each F2 population, twenty-six had identity greater than 91% and five presented identity of 24% to 44%. Among the twenty-six... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Daucus carota; Evolution; Molecular markers; SCAR. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000200013 |
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| Almeida-Ferreira, Giovanna Caputo; Cesumar; Oliveira, Alessandra Valéria de; CESUMAR; Prioli, Alberto José; UEM; Prioli, Sônia Maria Alves Pinto; UEM. |
| The introduction of exotic species in lake ecosystems has been greatly highlighted in the literature worldwide. Since introduction may threaten diversity of native fish, the issue turns up to be of paramount importance. Ecological effects may be predation, competition, parasitism or genetic, that is, changes in the genetic pool of populations owing to the occurrence of hybrids. Although the Tucunaré fish (Cichla) is native to the Amazon region, it can be found in other hydrographic basins in which it has been introduced. RAPD molecular marker research showed that there are two species (Cichla kelberi and C. piquiti) belonging to the genus Cichla in the rivers of the Paraná basin. Different morphotypes in the region may also be due to hybridization. Current... |
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Palavras-chave: 2.02.00.00-5 Hybridization; Fish introduction; Molecular markers Genética Hybridization; Fish introduction; Molecular markers. |
| Ano: 2011 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/4855 |
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| Registros recuperados: 181 | |
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